Resuscitation of eleven-year VBNC Citrobacter

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Resuscitation of eleven-year VBNC Citrobacter.

Citrobacter freundii strain WA1 was stressed by incubation in seawater microcosms for eleven years. After two years of starvation, no culturable strain was observed. Incubation of samples in nutrient-rich broth medium not supplemented with growth factors, however, allowed resuscitation of VBNC cells so that subsequent plating yielded observable colonies for significantly extended periods of tim...

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ژورنال

عنوان ژورنال: Journal of Water and Health

سال: 2008

ISSN: 1477-8920,1996-7829

DOI: 10.2166/wh.2008.131